DNA microinjection has played a pivotal role in developing transgenic organisms. Though most such scientific tests have a very low success rate of 2 to 5%, we are in the process of ensuring a better success with microinjection with well-planned scientific attempts to deliver a higher success rate on Genetic transfer.
During the process of genetic transfer to developing an effective genetic model for the transgenic organism, we have discovered WPI MICRO-ePORE, an advanced to ol & technique that can ensure a much better success rate time and again. Micro e-Pore from the World Precision Instruments(WPI), USA is such a product that ensures a higher success rate.
Transgenesis, a process of introducing transgene into a living organism so that the organism will exhibit a novel property and transmit that property to its offspring. It can be aided by plasmid vectors, liposomes, pronuclear injection, protoplast fusion, and ballistic DNA injection. It involves the use of recombinant DNA techniques
.
Foreign DNA is presented into the animal, after that, it must be transferred through the germ line so that every cell of the animal contains the same adapted genetic material. So, It is basically a genetic transfer process. This is done by isolating and copying the genetic material of interest. WPI MICRO-ePORE works as DNA Microinjection has wider benefits. Deoxyribonucleic Acid is usually inserted into animal cells using microinjection via the cell’s nuclear envelope directly into the nucleus. In this process, WPI MICRO-ePORE increased the viability of injected embryos. It has many flexible systems. It integrates with WPI ‘s PV820/PV830, as well as microinjection systems, like the Eppen Femtojet and the Narishige Injectors. It is easy to operate, hands-free operation with foot switch control. One can use this microinjection for pre and post-implantation in embryos of various species like mice, rodents, monkey, bovine, pig, zebrafish, etc. Transgenic organisms are able to direct foreign genes due to genetic code is similar for all organisms. WPI MICRO-ePORE is a small portable unit saves precious benchtop space. It works with major all inverted microscope, This microinjection is of diverse compounds and biomolecules- DNA, RNA, Proteins. It is the higher yield, saves time & low cost. Successfully tested using CRISPR-Cas9, the most current & promising technique. It offers complete solution for microinjection system & other research products.
Transgenic animals are having a wide range of advantages. Some of those are mentioned herewith:
a) Leaner meat:
Leaner meats are good for a low-fat diet. It is a reliable source of protein and has lower calories than non-lean meats.
b) Increased growth rates:
The production of transgenic livestock has been influential in providing new perceptions into the mechanisms of genetic transfer concerned in the control of growth. It is aided by manipulating growth factors, growth factor receptors, and growth modulators of DNA. In this dynamic world, the production of transgenic animals helps to balance the ever-growing demand with supply.
c) Improved disease resistance:
The production of transgenic animals has the opportunity to significantly improve animal health, enhance nutrition, protect the environment, increase animal welfare, and decrease animal’s disease. It forms disease resistance animals such as influenza-resistant pigs.
d) Improved food-conversion rates:
The transgenic livestock offers more quantity and better quality of food supply. Therefore higher food conversion is made possible.
e) Increased muscle mass:
The transgenic animal has increased muscle mass that helps to meet the growing demand of food. As an example, developed transgenic pigs and cattle can abate the high demand for products.
f) Improved wool quality:
Wool is a natural animal fiber popular in the production of outer clothing and is a vital source of income for several countries. Transgenic technology improves the efficiency of production of wool and its quality.
g) Improved nutritional quality:
Nutritional quality is a rising concern for better health and hygiene. In this regard the transgenic animal provides better nutrition, ensuring sound health with more resistance to disease.
h) Milk production:
The advance method of transgenic technology provides the opportunity to produce entirely new proteins in milk. In this way the survivability and improvement of animals are possible. So, a transgenic cow is capable of producing much greater quantity and quality of milk than a natural cow.